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Evanescence in emission: seeing fluorescence from supercritical angles

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Supercritical angle microscopy and spectroscopy
Authors: Martin Oheim, Adi Salomon, Maia Brunstein
Abstract: Fluorescence detection, either involving propagating or near-field emission, is widely being used in spectroscopy, sensing and microscopy. Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent (near-) field and it is a popular contrast generator for surface-selective fluorescence assays. Its emission equivalent, supercritical angle fluorescence (SAF) is co… ▽ More

Accepted by Biophys J.

Submitted 15 January, 2020; originally announced January 2020.