Team 4 – Membrane dynamics
We wish to understand how membrane trafficking and membrane transport processes shape the maintenance and adaptive homeostasis of mammalian cells, with a strong but not exclusive interest in neurons and synapses. We are particularly interested in two cell compartments, the lysosome and the dendritic spine.
Former description:
The nervous system is exquisitely compartmentalized at multiple levels. At biochemical level, this compartmentalization involves the sequestration and clearance of ions, signaling molecules and metabolites by transmembrane import and export into/from specific neural cells and, within cells, into/from specific organelles. These transport processes are performed by membrane channels and transporters, which are often essential for the function and health of the nervous system.
Our research focuses on intracellular transporters: (i) neurotransmitter transporters from synaptic vesicles and (ii) metabolite transporters from lysosomes and endosomes.
Neurotransmitter transporters are essential for the operation and modulation of synaptic transmission. On the other hand, lysosomal transporters participate in the degradation of damaged or aging components in the endocytic and autophagic pathways, a catabolic process which is essential for the survival and maintenance of eukaryotic cells, in particular long-lived postmitotic cells such as neurons. Lysosomal transporters terminate this process by exporting building blocks released from degraded macromolecules and organelles for subsequent reuse in biosynthetic pathways. They also represent the final effectors of metabolite homeostasis in the autophagic response to nutrient starvation. Because lysosomal membrane transport still represents a significant knowledge gap in cell biology, we also study these proteins and their dysfunction in non-neural cells.
Current projects include:
- Identification and characterization of novel lysosomal transporters
- Pathogenesis and therapy of lysosomal diseases
- Application of optical approaches to the study of small-molecule transport processes in their natural settings
Team leader
Bruno GASNIER, Senior Research Scientist, PI, CNRS, Team 4 - Membrane dynamicsbruno.gasnier@-Code to remove to avoid SPAM-parisdescartes.fr, +33 1 70 64 99 15, room 626
Members
Christine ANNE-LONGIN, Research Scientist, INSERM, Team 4 - Membrane dynamicschristine.anne@-Code to remove to avoid SPAM-parisdescartes.fr, +33 1 70 64 99 28, room 616
Damien CARREL, Assistant professor, Paris Descartes, Team 4 - Membrane dynamicsdamien.carrel@-Code to remove to avoid SPAM-parisdescartes.fr, +33 1 42 86 01 70 64 99 12, room 626
Rossella CONTI, Postdoctoral Researcher, Team 4 - Membrane dynamicsrossella.conti@-Code to remove to avoid SPAM-parisdescartes.fr, +33 1 70 64 99 28, room 626
Cécile DEBACKER, Assistant engineer, CNRS, Team 4 - Membrane dynamicscecile.jouffret@-Code to remove to avoid SPAM-parisdescartes.fr, +33 1 70 64 99 30, room 616
Claire DESNOS, Research Scientist, CNRS, Team 4 - Membrane dynamicsclaire.desnos@-Code to remove to avoid SPAM-parisdescartes.fr, +33 1 70 64 99 19, room 626
Isabelle FANGET, Engineer, CNRS, Team 4 - Membrane dynamicsisabelle.fanget@-Code to remove to avoid SPAM-parisdescartes.fr, +33 1 70 64 99 18, room 626
Océane GUELLE, Doctoral Student, Team 4 - Membrane dynamicsoceane.guelle@-Code to remove to avoid SPAM-parisdescartes.fr, +33 1 70 64 99 29, room 616
Solène LEBRUN, Assistant engineer, CNRS, Team 4 - Membrane dynamicssolene.lebrun@-Code to remove to avoid SPAM-parisdescartes.fr, +33 1 70 64 99 23, room 626- Amene MAJIDPUR, Master Student, Team 4 - Membrane dynamicsamene.majidipur@-Code to remove to avoid SPAM-parisdescartes.fr, +33 1 70 64 99 31, room 616
- Duc Anh NGO, Master Student, Team 4 - Membrane dynamicsduc-anh.ngo@-Code to remove to avoid SPAM-parisdescartes.fr, +33 1 70 64 99 23, room 626
Corinne SAGNÉ, Research Scientist, INSERM, Team 4 - Membrane dynamicscorinne.sagne@-Code to remove to avoid SPAM-parisdescartes.fr, +33 1 70 64 99 16, room 616
Yann TERRES, Doctoral Student, Team 4 - Membrane dynamicsyann.terres@-Code to remove to avoid SPAM-parisdescartes.fr, +33 1 70 64 99 27, room 626
Recent Publications
Articles
- Cortical Neuron Migration and Dendrite Morphology are Regulated by Carboxypeptidase E.
Liang C, Carrel D, Omelchenko A, Kim H, Patel A, Fanget I, Firestein BL : Cereb Cortex, 2018 - Increased signaling by the autism-related Engrailed-2 protein enhances dendritic branching and spine density, alters synaptic structural matching, and exaggerates protein synthesis.
Soltani A, Lebrun S, Carpentier G, Zunino G, Chantepie S, Maïza A, Bozzi Y, Desnos C, Darchen F, Stettler O : PLOS ONE, 2017 - Meeting after meeting: 20 years of discoveries by the members of the Exocytosis-Endocytosis Club.
Niedergang F, Gasman S, Vitale N, Desnos C, Lamaze C : Biol Cell, 2017 - Increased signaling by the autism-related Engrailed-2 protein enhances dendritic branching and spine density, alters synaptic structural matching, and exaggerates protein synthesis.
Soltani A, Lebrun S, Carpentier G, Zunino G, Chantepie S, Maïza A, Bozzi Y, Desnos C, Darchen F, Stettler O : PLoS One, 2017 - Submillisecond Optogenetic Control of Neuronal Firing with Two-Photon Holographic Photoactivation of Chronos.
Ronzitti E, Conti R, Zampini V, Tanese D, Foust AJ, Klapoetke N, Boyden ES, Papagiakoumou E, Emiliani V : J Neurosci, 2017 - Clinical or ATPase domain mutations in ABCD4 disrupt the interaction between the vitamin B-trafficking proteins ABCD4 and LMBD1.
Fettelschoss V, Burda P, Sagné C, Coelho D, De Laet C, Lutz S, Suormala T, Fowler B, Pietrancosta N, Gasnier B, Bornhauser B, Froese DS, Baumgartner MR : J Biol Chem, 2017 - SNAT7 is the primary lysosomal glutamine exporter required for extracellular protein-dependent growth of cancer cells.
Verdon Q, Boonen M, Ribes C, Jadot M, Gasnier B, Sagné C : Proc Natl Acad Sci U S A, 2017 - Superresolving dendritic spine morphology with STED microscopy under holographic photostimulation.
Lauterbach MA, Guillon M, Desnos C, Khamsing D, Jaffal Z, Darchen F, Emiliani V : Neurophotonics, 2016 - Computer Generated Holography with Intensity-Graded Patterns.
Conti R, Assayag O, de Sars V, Guillon M, Emiliani V : Front Cell Neurosci, 2016 - Watching the Pulleys Turn while the Elevator Moves in a Secondary Transporter.
Gasnier B : Biophys J, 2016 - Polarity protein Par3 controls B-cell receptor dynamics and antigen extraction at the immune synapse.
Reversat A, Yuseff MI, Lankar D, Malbec O, Obino D, Maurin M, Penmatcha NV, Amoroso A, Sengmanivong L, Gundersen GG, Mellman I, Darchen F, Desnos C, Pierobon P, Lennon-Duménil AM : Mol Biol Cell, 2015 - TMEM175 Is an Organelle K(+) Channel Regulating Lysosomal Function.
Cang C, Aranda K, Seo YJ, Gasnier B, Ren D : Cell, 2015 - Cdc42 controls the dilation of the exocytotic fusion pore by regulating membrane tension.
Bretou M, Jouannot O, Fanget I, Pierobon P, Larochette N, Gestraud P, Guillon M, Emiliani V, Gasman S, Desnos C, Lennon-Duménil AM, Darchen F : Mol Biol Cell, 2014 - Vesicular neurotransmitter transporters: mechanistic aspects.
Anne C, Gasnier B : Curr Top Membr, 2014