About half of the cells in the human brain are not neurons. Our aim is to understand the contribution of glial cells and particularly that of cortical astrocytes and Bergmann glia of the cerebellum to brain metabolism and signaling. We focus on how ion channels, transporters and organelles of the glial perisynaptic processes ensheathing neuronal synapses contribute to shaping and modulating synaptic transmission. Members of the ‘Biophysics of the Brain’ team work together to develop new optical tools for super-resolution and 3-D tissue imaging of glial morphology and physiology. We use various linear and non-linear microscopy techniques together with immunofluorescence, electrophysiology and advanced mouse genetics to crack the astrocyte calcium code. Our biological preparations range from cell culture, brain slices and hiPSC-derived 3-D organoids to mice, in vivo. A new line of research focusses on tissue clarification and 1- and 2-photon light-sheet imaging.
Team leader
Martin Oheim, Director of SPPIN, Senior Research Scientist, PI, CNRS, Core unit, Team 1 - Biophysics of the brainmartin.oheim@-Code to remove to avoid SPAM-u-paris.fr, +33 1 70 64 99 14 (secretariat), room E360
Members
Vincent Armand, Assistant Professor, University of Paris, Team 1 - Biophysics of the brainvincent.armand@-Code to remove to avoid SPAM-u-paris.fr, +33 1 42 86 42 21, room P368
Brigitte Delhomme, Engineer, CNRS, Team 1 - Biophysics of the brainbrigitte.delhomme@-Code to remove to avoid SPAM-u-paris.fr, +33 1 76 53 42 13, room P374A
Diane Derrien, Engineering Assistant, CNRS, Team 1 - Biophysics of the braindiane.derrien@-Code to remove to avoid SPAM-u-paris.fr, +33 1 76 53 42 13, room P376
Philippe Djian, Senior Research Scientist, CNRS, Team 1 - Biophysics of the brainphilippe.djian@-Code to remove to avoid SPAM-u-paris.fr, +33 1 76 53 42 10
Carine Julien, Assistant Professor, Team 1 - Biophysics of the braincarine.julien@-Code to remove to avoid SPAM-u-paris.fr
Natalia Mañas Chavernas, Master Student, Team 1 - Biophysics of the brainnatalia.manas-chavernas@-Code to remove to avoid SPAM-etu.u-paris.fr
Clément Ricard, Assistant Professor, University of Paris, Team 1 - Biophysics of the brainclement.ricard@-Code to remove to avoid SPAM-u-paris.fr, room H344
Marc Saghiah, Master Student, Team 1 - Biophysics of the brainmarc.saghiah@-Code to remove to avoid SPAM-etu.u-paris.fr, +33 1 42 86 42 21, room P368
Adi Salomon, Chateaubriand Senior Research Fellow, Team 1 - Biophysics of the brainadi.salomon@-Code to remove to avoid SPAM-biu.ac.il, +33 1 76 53 42 11, room H353
Antonin Singer, PhD Student, Team 1 - Biophysics of the brainantonin.singer@-Code to remove to avoid SPAM-u-paris.fr, +33 1 42 86 42 21, room P368
Kaitlin Szederkenyi, PhD Student, Team 1 - Biophysics of the brainkaitlin.szederkenyi@-Code to remove to avoid SPAM-u-paris.fr, +33 1 42 86 42 21, room P368
Recent Publications
Articles
- Optometry for a short-sighted microscope.
Julien C, Oheim M : Biophys J, 2021 - A role for BDNF- and NMDAR-induced lysosomal recruitment of mTORC1 in the regulation of neuronal mTORC1 activity.
Khamsing D, Lebrun S, Fanget I, Larochette N, Tourain C, de Sars V, Brunstein M, Oheim M, Carrel D, Darchen F, Desnos C : Mol Brain, 2021 - A simple, inexpensive and multi-scale 3-D fluorescent test sample for optical sectioning microscopies.
Olevsko I, Szederkenyi K, Corridon J, Au A, Delhomme B, Bastien T, Fernandes J, Yip C, Oheim M, Salomon A : Microsc Res Tech, 2021 - Astrocytes respond to a neurotoxic Aβ fragment with state-dependent Ca alteration and multiphasic transmitter release.
Pham C, Hérault K, Oheim M, Maldera S, Vialou V, Cauli B, Li D : Acta Neuropathol Commun, 2021 - Pulse-length effects in 2P-micoscopy. I. Infrared excitation induces brain heating and calcium microdomain hyperactivity in cortical astrocytes.
Schmidt E, Oheim M : Biophys J, 2020 - Cathodoluminescence Nanoscopy of 3D Plasmonic Networks
Racheli Ron, Marcin Stefan Zielinski, Adi Salomon : Nano Lett, 2020 - Supercritical angle fluorescence microscopy and spectroscopy (PDF)
Oheim M, Salomon A, Brunstein M : Biophys J, 2020 - Fabrication of dipole-aligned thin films of Porphyrin J-aggregates over large surfaces (PDF)
Adam Weissman, Hodaya Klimovsky, Dor Harel, Racheli Ron, Martin Oheim, Adi Salomon : Langmuir, 2020 - Fast 3-D Imaging of Brain Organoids With a New Single-Objective Planar-Illumination Two-Photon Microscope.
Rakotoson I, Delhomme B, Djian P, Deeg A, Brunstein M, Seebacher C, Uhl R, Ricard C, Oheim M : Front Neuroanat, 2019 - Calibrating evanescent-wave penetration depths for biological TIRF microscopy (PDF)
Martin Oheim, Adi Salomon, Adam Weissman, Maia Brunstein, Ute Becherer : Biophys J, 2019 - Synthesis of Bio-inspired Photocatalytic Ruthenium Complexes; their Optical Properties and Solvatochromic Effect
Weissman A, Amir D, Elias Y, Pinkas I, Mathias J-L, Benisvy L, Salomon A : Chemphyschem, 2018 - Nanoporous Metallic Networks: Fabrication, Optical Properties and Applications
Ron R Haleva E, Salomon A : Advanced materials, 2018 - Second Harmonic Generation Hot-Spot on a Centrosymmetric Smooth Silver Surface
Galanty M, Shavit O, Aharon H, Gachet D, Salomon A : Light: Science and application, 2018 - Decoding the Information Contained in Fluorophore Radiation Patterns
Maia Brunstein, Adi Salomon, Martin Oheim : ACS Nano, 2018 - Quantifying Colocalisation in Biological Fluorescence Microscopy
Martin Oheim, Maia Brunstein : ELS, 2018 - Fast, multicolor 3-D imaging of brain organoids with a new single-objective two-photon virtual light-sheet microscope
Irina Rakotoson, Brigitte Delhomme, Philippe Djian, Andreas Deeg, Maia Brunstein, Christian Seebacher, Rainer Uhl, Clement Ricard, Martin Oheim : BioRxiv, 2018 - Two-photon imaging induces brain heating and calcium microdomain hyper-activity in cortical astrocytes
Elke M Schmidt, Martin Oheim : BioRxiv, 2018 - Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells.
Ricard C, Arroyo ED, He CX, Portera-Cailliau C, Lepousez G, Canepari M, Fiole D : Brain Struct Funct, 2018 - Near-Membrane Refractometry Using Supercritical Angle Fluorescence. (PDF)
Brunstein M, Roy L, Oheim M : Biophys J, 2017 - Local energy on demand: Are spontaneous astrocytic Ca2+-microdomains the regulartory unit for astrocyte-neuron metabolic cooperation?
Oheim M, Schmidt E, Hirrlinger J : Brain Res Bull, 2017 - Phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice.
Ricard C, Tchoghandjian A, Luche H, Grenot P, Figarella-Branger D, Rougon G, Malissen M, Debarbieux F : Sci Rep, 2016 - Combination of an optical parametric oscillator and quantum-dots 655 to improve imaging depth of vasculature by intravital multicolor two-photon microscopy.
Ricard C, Lamasse L, Jaouen A, Rougon G, Debarbieux F : Biomed Opt Express, 2016 - Microglial Janus kinase/signal transduction and activator of transcription 3 pathway activity directly impacts astrocyte and spinal neuron characteristics.
Molet J, Mauborgne A, Diallo M, Armand V, Geny D, Villanueva L, Boucher Y, Pohl M : J Neurochem, 2016 - GABAB receptor cell-surface export is controlled by an endoplasmic reticulum gatekeeper.
Doly S, Shirvani H, Gäta G, Meye FJ, Emerit MB, Enslen H, Achour L, Pardo-Lopez L, Yang SK, Armand V, Gardette R, Giros B, Gassmann M, Bettler B, Mameli M, Darmon M, Marullo S : Mol Psychiatry, 2016 - Dependence of descriptors of co-localization on microscope spatiotemporal resolution and the choice of regions of interest.
Brunstein M, Oheim M : Microsc Res Tech, 2016 - The importance of basonuclin 2 in adult mice and its relation to basonuclin 1.
Vanhoutteghem A, Delhomme B, Hervé F, Nondier I, Petit JM, Araki M, Araki K, Djian P : Mech Dev, 2016 - TIRF (Total Internal Reflection Fluorescence)
Martin Oheim : ELS, 2016
Speech communication
- Nanostructured substrates for super-resolution imaging (proceeding)
Maia Brunstein, Anne Talneau, Minh-Chau Nguyen, Pascal Berto, Anne-Laure Fehrembach, Anne Sentenac, Martin Oheim : Imaging Systems and Applications 2019, Munich Germany, 2019
Patents
- Calibration standard for evanescence microscopy
Martin Oheim, Adi Salomon : Patent # : WO 2020/053367 A1, 2020 - Micron-size plasmonic color sorter
A Salomon, A Weissman, E Segal : Patent # : PCT/IL2017/050756, (US 2018008029A1), 2016