About half of the cells in the human brain are not neurons. Our aim is to understand the contribution of glial cells and particularly that of cortical astrocytes and Bergmann glia of the cerebellum to brain metabolism and signaling. We focus on how ion channels, transporters and organelles of the glial perisynaptic processes ensheathing neuronal synapses contribute to shaping and modulating synaptic transmission. Members of the ‘Biophysics of the Brain’ team work together to develop new optical tools for super-resolution and 3-D tissue imaging of glial morphology and physiology. We use various linear and non-linear microscopy techniques together with immunofluorescence, electrophysiology and advanced mouse genetics to crack the astrocyte calcium code. Our biological preparations range from cell culture, brain slices and hiPSC-derived 3-D organoids to mice, in vivo. A new line of research focusses on tissue clarification and 1- and 2-photon light-sheet imaging.

Recent Publications

Articles

• Pulse-length effects in 2P-micoscopy. I. Infrared excitation induces brain heating and calcium microdomain hyperactivity in cortical astrocytes.
  Schmidt E, Oheim M : Biophys J, 2020
• Cathodoluminescence Nanoscopy of 3D Plasmonic Networks
  Racheli Ron, Marcin Stefan Zielinski, Adi Salomon : Nano Lett, 2020
• Chromato-axial memory effect through aforward-scattering slab
  Longdi Zhu, Jacques Boutet de Monvel, Pascal Berto, Sophie Brasselet, Sylvain Gigan, Marc Guillon : Optica, 2020
• Supercritical angle fluorescence microscopy and spectroscopy (PDF)
  Oheim M, Salomon A, Brunstein M : Biophys J, 2020
• Fabrication of dipole-aligned thin films of Porphyrin J-aggregates over large surfaces (PDF)
  Adam Weissman, Hodaya Klimovsky, Dor Harel, Racheli Ron, Martin Oheim, Adi Salomon : Langmuir, 2020
• Reconfigurable Temperature Control at the Microscale by Light Shaping
  Chang Liu, Gilles Tessier, Sergio Iván Flores Esparza, Marc Guillon, Pascal Berto : ACS Photonics, 2019
• Fast 3-D Imaging of Brain Organoids With a New Single-Objective Planar-Illumination Two-Photon Microscope.
  Rakotoson I, Delhomme B, Djian P, Deeg A, Brunstein M, Seebacher C, Uhl R, Ricard C, Oheim M : Front Neuroanat, 2019
• Focusing large spectral bandwidths through scattering media
  A G Vesga, M Hofer, NK Balla, HB De Aguiar, M Guillon, S Brasselet : Opt Express, 2019
• ATP6AP2 variant impairs CNS development and neuronal survival to cause fulminant neurodegeneration.
  Hirose T, Cabrera-Socorro A, Chitayat D, Lemonnier T, Féraud O, Cifuentes-Diaz C, Gervasi N, Mombereau C, Ghosh T, Stoica L, Bacha JDA, Yamada H, Lauterbach MA, Guillon M, Kaneko K, Norris JW, Siriwardena K, Blasér S, Teillon J, Mendoza-Londono R, Russeau M, Hadoux J, Ito S, Corvol P, Matheus MG, Holden KR, Takei K, Emiliani V, Bennaceur-Griscelli A, Schwartz CE, Nguyen G, Groszer M : J Clin Invest, 2019
• Topological transformation of speckles by symmetry control and spiral wavefront modulation
  Jerome Gateau, Ferdinand Claude, Gilles Tessier, Marc Guillon : Optica, 2019
• Compressive three-dimensional super-resolution microscopy with speckle-saturated fluorescence excitation
  M Pascucci, S Ganesan, A Tripathi, O Katz, V Emiliani, M Guillon : Nat Commun, 2019
• Calibrating evanescent-wave penetration depths for biological TIRF microscopy (PDF)
  Martin Oheim, Adi Salomon, Adam Weissman, Maia Brunstein, Ute Becherer : Biophys J, 2019
• Synthesis of Bio-inspired Photocatalytic Ruthenium Complexes; their Optical Properties and Solvatochromic Effect
  Weissman A, Amir D, Elias Y, Pinkas I, Mathias J-L, Benisvy L, Salomon A : Chemphyschem, 2018
• Nanoporous Metallic Networks: Fabrication, Optical Properties and Applications
  Ron R Haleva E, Salomon A : Advanced materials, 2018
• Second Harmonic Generation Hot-Spot on a Centrosymmetric Smooth Silver Surface
  Galanty M, Shavit O, Aharon H, Gachet D, Salomon A : Light: Science and application, 2018
• Wrapping-free numerical refocusing of scalar electromagnetic fields.
  Berto P, Guillon M, Bon P : Appl Opt, 2018
• Decoding the Information Contained in Fluorophore Radiation Patterns
  Maia Brunstein, Adi Salomon, Martin Oheim : ACS Nano, 2018
• Quantifying Colocalisation in Biological Fluorescence Microscopy
  Martin Oheim, Maia Brunstein : ELS, 2018
• Fast, multicolor 3-D imaging of brain organoids with a new single-objective two-photon virtual light-sheet microscope
  Irina Rakotoson, Brigitte Delhomme, Philippe Djian, Andreas Deeg, Maia Brunstein, Christian Seebacher, Rainer Uhl, Clement Ricard, Martin Oheim : BioRxiv, 2018
• Two-photon imaging induces brain heating and calcium microdomain hyper-activity in cortical astrocytes
  Elke M Schmidt, Martin Oheim : BioRxiv, 2018
• Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells.
  Ricard C, Arroyo ED, He CX, Portera-Cailliau C, Lepousez G, Canepari M, Fiole D : Brain Struct Funct, 2018
• Complementary Speckle Patterns: Deterministic Interchange of Intrinsic Vortices and Maxima through Scattering Media.
  Gateau J, Rigneault H, Guillon M : Phys Rev Lett, 2017
• Vortex-free phase profiles for uniform patterning with computer-generated holography.
  Guillon M, Forget BC, Foust AJ, De Sars V, Ritsch-Marte M, Emiliani V : Opt Express, 2017
• Otoferlin acts as a Ca sensor for vesicle fusion and vesicle pool replenishment at auditory hair cell ribbon synapses.
  Michalski N, Goutman JD, Auclair SM, Boutet de Monvel J, Tertrais M, Emptoz A, Parrin A, Nouaille S, Guillon M, Sachse M, Ciric D, Bahloul A, Hardelin JP, Sutton RB, Avan P, Krishnakumar SS, Rothman JE, Dulon D, Safieddine S, Petit C : Elife, 2017
• Wavefront sensing with a thin diffuser.
  Berto P, Rigneault H, Guillon M : Opt Lett, 2017
• Near-Membrane Refractometry Using Supercritical Angle Fluorescence. (PDF)
  Brunstein M, Roy L, Oheim M : Biophys J, 2017
• Local energy on demand: Are spontaneous astrocytic Ca2+-microdomains the regulartory unit for astrocyte-neuron metabolic cooperation?
  Oheim M, Schmidt E, Hirrlinger J : Brain Res Bull, 2017
• Curcumin (Diferulolylmethane) Reduces Transglutaminase 2 Overexpression Induced by Retinoic Acid in Human Nervous Cell Lines.
  Gatta NG, Cammarota G, Iannaccone M, Serretiello E, Gentile V : Neuroimmunomodulation, 2016
• Superresolution Imaging of Optical Vortices in a Speckle Pattern.
  Pascucci M, Tessier G, Emiliani V, Guillon M : Phys Rev Lett, 2016
• Superresolving dendritic spine morphology with STED microscopy under holographic photostimulation.
  Lauterbach MA, Guillon M, Desnos C, Khamsing D, Jaffal Z, Darchen F, Emiliani V : Neurophotonics, 2016
• Computer Generated Holography with Intensity-Graded Patterns.
  Conti R, Assayag O, de Sars V, Guillon M, Emiliani V : Front Cell Neurosci, 2016
• Phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice.
  Ricard C, Tchoghandjian A, Luche H, Grenot P, Figarella-Branger D, Rougon G, Malissen M, Debarbieux F : Sci Rep, 2016
• Combination of an optical parametric oscillator and quantum-dots 655 to improve imaging depth of vasculature by intravital multicolor two-photon microscopy.
  Ricard C, Lamasse L, Jaouen A, Rougon G, Debarbieux F : Biomed Opt Express, 2016
• Microglial Janus kinase/signal transduction and activator of transcription 3 pathway activity directly impacts astrocyte and spinal neuron characteristics.
  Molet J, Mauborgne A, Diallo M, Armand V, Geny D, Villanueva L, Boucher Y, Pohl M : J Neurochem, 2016
• GABAB receptor cell-surface export is controlled by an endoplasmic reticulum gatekeeper.
  Doly S, Shirvani H, Gäta G, Meye FJ, Emerit MB, Enslen H, Achour L, Pardo-Lopez L, Yang SK, Armand V, Gardette R, Giros B, Gassmann M, Bettler B, Mameli M, Darmon M, Marullo S : Mol Psychiatry, 2016
• Dependence of descriptors of co-localization on microscope spatiotemporal resolution and the choice of regions of interest.
  Brunstein M, Oheim M : Microsc Res Tech, 2016
• The importance of basonuclin 2 in adult mice and its relation to basonuclin 1.
  Vanhoutteghem A, Delhomme B, Hervé F, Nondier I, Petit JM, Araki M, Araki K, Djian P : Mech Dev, 2016
• TIRF (Total Internal Reflection Fluorescence)
  Martin Oheim : ELS, 2016

Speech communication

• Nanostructured substrates for super-resolution imaging (proceeding)
  Maia Brunstein, Anne Talneau, Minh-Chau Nguyen, Pascal Berto, Anne-Laure Fehrembach, Anne Sentenac, Martin Oheim : Imaging Systems and Applications 2019, Munich Germany, 2019

Patents

• Calibration standard for evanescence microscopy
  Martin Oheim, Adi Salomon : Patent # : WO 2020/053367 A1, 2020
• Micron-size plasmonic color sorter
  A Salomon, A Weissman, E Segal : Patent # : PCT/IL2017/050756, (US 2018008029A1), 2016