Team 1 – Biophysics of the brain
About half of the cells in the human brain are not neurons. Our aim is to understand the contribution of glial cells and particularly that of cortical astrocytes and Bergmann glia of the cerebellum to brain metabolism and signaling. We focus on how ion channels, transporters and organelles of the glial perisynaptic processes ensheathing neuronal synapses contribute to shaping and modulating synaptic transmission. Members of the ‘Biophysics of the Brain’ team work together to develop new optical tools for super-resolution and 3-D tissue imaging of glial morphology and physiology. We use various linear and non-linear microscopy techniques together with immunofluorescence, electrophysiology and advanced mouse genetics to crack the astrocyte calcium code. Our biological preparations range from cell culture, brain slices and hiPSC-derived 3-D organoids to mice, in vivo. A new line of research focusses on tissue clarification and 1- and 2-photon light-sheet imaging.
Team leader
- Martin OHEIM, Director of SPPIN, Senior Research Scientist, PI, CNRS, Core unit, Team 1 - Biophysics of the brainmartin.oheim@-Code to remove to avoid SPAM-u-paris.fr, +33 1 70 64 99 14 (secretariat), room E360
Members
- Payvand ARJMAND, PhD Student, Team 1 - Biophysics of the brainpayvand.arjmand@-Code to remove to avoid SPAM-u-paris.fr, +33 1 76 53 43 49, room P362
- Vincent ARMAND, Assistant Professor, University of Paris, Team 1 - Biophysics of the brainvincent.armand@-Code to remove to avoid SPAM-u-paris.fr, +33 1 42 86 42 21, room P368
- Brigitte DELHOMME, Engineer, CNRS, Team 1 - Biophysics of the brainbrigitte.delhomme@-Code to remove to avoid SPAM-u-paris.fr, +33 1 76 53 42 13, room P374A
- Diane DERRIEN, Engineering Assistant, CNRS, Team 1 - Biophysics of the braindiane.derrien@-Code to remove to avoid SPAM-u-paris.fr, +33 1 76 53 42 13, room P376
- Philippe DJIAN, Senior Research Scientist, CNRS, Team 1 - Biophysics of the brainphilippe.djian@-Code to remove to avoid SPAM-u-paris.fr, +33 1 76 53 42 10
- Nicola Gaetano GATTA, Phd visitor, Team 1 - Biophysics of the brainnicolagaetano.gatta@-Code to remove to avoid SPAM-unicampania.it, +33 1 42 86 20 94, room P374A
- Marc GUILLON, Assistant Professor, University of Paris, Team 1 - Biophysics of the brainmarc.guillon@-Code to remove to avoid SPAM-u-paris.fr, +33 1 76 53 43 49, room P362
- Clément RICARD, Assistant Professor, University of Paris, Team 1 - Biophysics of the brainclement.ricard@-Code to remove to avoid SPAM-u-paris.fr, +33 1 76 53 43 49, room P362
- Adi SALOMON, Chateaubriand Senior Research Fellow, Team 1 - Biophysics of the brainadi.salomon@-Code to remove to avoid SPAM-biu.ac.il, +33 1 76 53 42 11, room H353
- Antonin SINGER, PhD Student, Team 1 - Biophysics of the brainantonin.singer@-Code to remove to avoid SPAM-u-paris.fr, +33 1 42 86 42 21, room P368
- Kaitlin SZEDERKENYI, PhD Student, Team 1 - Biophysics of the brainkaitlin.szederkenyi@-Code to remove to avoid SPAM-u-paris.fr, +33 1 42 86 42 21, room P368
- Tengfei WU, Postdoctoral Researcher, Team 1 - Biophysics of the braintengfei.wu@-Code to remove to avoid SPAM-u-paris.fr, +33 1 76 53 43 49, room P362
Recent Publications
Articles
- Three-dimensional broadband light beam manipulation in forward scattering samples
Payvand Arjmand, Ori Katz, Sylvain Gigan, Marc Guillon : Opt Express, 2021 - Color imaging through scattering media based on phase retrieval with triple correlation
Zhu L, Wu Y, Liu J, Wu T, Liu L, Shao X : Optics and Lasers in Engineering, 2020 - Non-invasive single-shot recovery of a point-spread function of a memory effect based scattering imaging system.
Wu T, Dong J, Gigan S : Opt Lett, 2020 - Pulse-length effects in 2P-micoscopy. I. Infrared excitation induces brain heating and calcium microdomain hyperactivity in cortical astrocytes.
Schmidt E, Oheim M : Biophys J, 2020 - Cathodoluminescence Nanoscopy of 3D Plasmonic Networks
Racheli Ron, Marcin Stefan Zielinski, Adi Salomon : Nano Lett, 2020 - Chromato-axial memory effect through aforward-scattering slab
Longdi Zhu, Jacques Boutet de Monvel, Pascal Berto, Sophie Brasselet, Sylvain Gigan, Marc Guillon : Optica, 2020 - Supercritical angle fluorescence microscopy and spectroscopy (PDF)
Oheim M, Salomon A, Brunstein M : Biophys J, 2020 - Fabrication of dipole-aligned thin films of Porphyrin J-aggregates over large surfaces (PDF)
Adam Weissman, Hodaya Klimovsky, Dor Harel, Racheli Ron, Martin Oheim, Adi Salomon : Langmuir, 2020 - Imaging through scattering layers exceeding memory effect range by exploiting prior information
Guo C, Liu J, Li W, Wu T, Zhu L, Wang J, Wang G, and Shao X : Optics Communications, 2019 - Reconfigurable Temperature Control at the Microscale by Light Shaping
Chang Liu, Gilles Tessier, Sergio Iván Flores Esparza, Marc Guillon, Pascal Berto : ACS Photonics, 2019 - Fast 3-D Imaging of Brain Organoids With a New Single-Objective Planar-Illumination Two-Photon Microscope.
Rakotoson I, Delhomme B, Djian P, Deeg A, Brunstein M, Seebacher C, Uhl R, Ricard C, Oheim M : Front Neuroanat, 2019 - Focusing large spectral bandwidths through scattering media
A G Vesga, M Hofer, NK Balla, HB De Aguiar, M Guillon, S Brasselet : Opt Express, 2019 - ATP6AP2 variant impairs CNS development and neuronal survival to cause fulminant neurodegeneration.
Hirose T, Cabrera-Socorro A, Chitayat D, Lemonnier T, Féraud O, Cifuentes-Diaz C, Gervasi N, Mombereau C, Ghosh T, Stoica L, Bacha JDA, Yamada H, Lauterbach MA, Guillon M, Kaneko K, Norris JW, Siriwardena K, Blasér S, Teillon J, Mendoza-Londono R, Russeau M, Hadoux J, Ito S, Corvol P, Matheus MG, Holden KR, Takei K, Emiliani V, Bennaceur-Griscelli A, Schwartz CE, Nguyen G, Groszer M : J Clin Invest, 2019 - Topological transformation of speckles by symmetry control and spiral wavefront modulation
Jerome Gateau, Ferdinand Claude, Gilles Tessier, Marc Guillon : Optica, 2019 - Compressive three-dimensional super-resolution microscopy with speckle-saturated fluorescence excitation
M Pascucci, S Ganesan, A Tripathi, O Katz, V Emiliani, M Guillon : Nat Commun, 2019 - Calibrating evanescent-wave penetration depths for biological TIRF microscopy (PDF)
Martin Oheim, Adi Salomon, Adam Weissman, Maia Brunstein, Ute Becherer : Biophys J, 2019 - Non-invasive imaging through thin scattering layers with broadband illumination
Wu T, Guo C, Shao X : ArXiv preprint arXiv:180906854, 2018 - Recovering the spectral and spatial information of an object behind a scattering media
Zhu L, Liu J, Feng L, Guo C, Wu T, and Shao X : OSA Continuum, 2018 - Tracking moving targets behind a scattering medium via speckle correlation.
Guo C, Liu J, Wu T, Zhu L, Shao X : Appl Opt, 2018 - Synthesis of Bio-inspired Photocatalytic Ruthenium Complexes; their Optical Properties and Solvatochromic Effect
Weissman A, Amir D, Elias Y, Pinkas I, Mathias J-L, Benisvy L, Salomon A : Chemphyschem, 2018 - Nanoporous Metallic Networks: Fabrication, Optical Properties and Applications
Ron R Haleva E, Salomon A : Advanced materials, 2018 - Second Harmonic Generation Hot-Spot on a Centrosymmetric Smooth Silver Surface
Galanty M, Shavit O, Aharon H, Gachet D, Salomon A : Light: Science and application, 2018 - Wrapping-free numerical refocusing of scalar electromagnetic fields.
Berto P, Guillon M, Bon P : Appl Opt, 2018 - Decoding the Information Contained in Fluorophore Radiation Patterns
Maia Brunstein, Adi Salomon, Martin Oheim : ACS Nano, 2018 - Quantifying Colocalisation in Biological Fluorescence Microscopy
Martin Oheim, Maia Brunstein : ELS, 2018 - Fast, multicolor 3-D imaging of brain organoids with a new single-objective two-photon virtual light-sheet microscope
Irina Rakotoson, Brigitte Delhomme, Philippe Djian, Andreas Deeg, Maia Brunstein, Christian Seebacher, Rainer Uhl, Clement Ricard, Martin Oheim : BioRxiv, 2018 - Two-photon imaging induces brain heating and calcium microdomain hyper-activity in cortical astrocytes
Elke M Schmidt, Martin Oheim : BioRxiv, 2018 - Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells.
Ricard C, Arroyo ED, He CX, Portera-Cailliau C, Lepousez G, Canepari M, Fiole D : Brain Struct Funct, 2018 - Imaging through a thin scattering layer and jointly retrieving the point-spread-function using phase-diversity.
Wu T, Dong J, Shao X, Gigan S : Opt Express, 2017 - Complementary Speckle Patterns: Deterministic Interchange of Intrinsic Vortices and Maxima through Scattering Media.
Gateau J, Rigneault H, Guillon M : Phys Rev Lett, 2017 - Vortex-free phase profiles for uniform patterning with computer-generated holography.
Guillon M, Forget BC, Foust AJ, De Sars V, Ritsch-Marte M, Emiliani V : Opt Express, 2017 - Otoferlin acts as a Ca sensor for vesicle fusion and vesicle pool replenishment at auditory hair cell ribbon synapses.
Michalski N, Goutman JD, Auclair SM, Boutet de Monvel J, Tertrais M, Emptoz A, Parrin A, Nouaille S, Guillon M, Sachse M, Ciric D, Bahloul A, Hardelin JP, Sutton RB, Avan P, Krishnakumar SS, Rothman JE, Dulon D, Safieddine S, Petit C : Elife, 2017 - Wavefront sensing with a thin diffuser.
Berto P, Rigneault H, Guillon M : Opt Lett, 2017 - Near-Membrane Refractometry Using Supercritical Angle Fluorescence. (PDF)
Brunstein M, Roy L, Oheim M : Biophys J, 2017 - Local energy on demand: Are spontaneous astrocytic Ca2+-microdomains the regulartory unit for astrocyte-neuron metabolic cooperation?
Oheim M, Schmidt E, Hirrlinger J : Brain Res Bull, 2017 - Simulation and experimental verification for imaging of gray-scale objects through scattering layers.
Li H, Wu T, Liu J, Gong C, Shao X : Appl Opt, 2016 - Single-shot diffraction-limited imaging through scattering layers via bispectrum analysis.
Wu T, Katz O, Shao X, Gigan S : Opt Lett, 2016 - Curcumin (Diferulolylmethane) Reduces Transglutaminase 2 Overexpression Induced by Retinoic Acid in Human Nervous Cell Lines.
Gatta NG, Cammarota G, Iannaccone M, Serretiello E, Gentile V : Neuroimmunomodulation, 2016 - Superresolution Imaging of Optical Vortices in a Speckle Pattern.
Pascucci M, Tessier G, Emiliani V, Guillon M : Phys Rev Lett, 2016 - Superresolving dendritic spine morphology with STED microscopy under holographic photostimulation.
Lauterbach MA, Guillon M, Desnos C, Khamsing D, Jaffal Z, Darchen F, Emiliani V : Neurophotonics, 2016 - Computer Generated Holography with Intensity-Graded Patterns.
Conti R, Assayag O, de Sars V, Guillon M, Emiliani V : Front Cell Neurosci, 2016 - Phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice.
Ricard C, Tchoghandjian A, Luche H, Grenot P, Figarella-Branger D, Rougon G, Malissen M, Debarbieux F : Sci Rep, 2016 - Combination of an optical parametric oscillator and quantum-dots 655 to improve imaging depth of vasculature by intravital multicolor two-photon microscopy.
Ricard C, Lamasse L, Jaouen A, Rougon G, Debarbieux F : Biomed Opt Express, 2016 - Microglial Janus kinase/signal transduction and activator of transcription 3 pathway activity directly impacts astrocyte and spinal neuron characteristics.
Molet J, Mauborgne A, Diallo M, Armand V, Geny D, Villanueva L, Boucher Y, Pohl M : J Neurochem, 2016 - GABAB receptor cell-surface export is controlled by an endoplasmic reticulum gatekeeper.
Doly S, Shirvani H, Gäta G, Meye FJ, Emerit MB, Enslen H, Achour L, Pardo-Lopez L, Yang SK, Armand V, Gardette R, Giros B, Gassmann M, Bettler B, Mameli M, Darmon M, Marullo S : Mol Psychiatry, 2016 - Dependence of descriptors of co-localization on microscope spatiotemporal resolution and the choice of regions of interest.
Brunstein M, Oheim M : Microsc Res Tech, 2016 - The importance of basonuclin 2 in adult mice and its relation to basonuclin 1.
Vanhoutteghem A, Delhomme B, Hervé F, Nondier I, Petit JM, Araki M, Araki K, Djian P : Mech Dev, 2016 - TIRF (Total Internal Reflection Fluorescence)
Martin Oheim : ELS, 2016
Speech communication
- Nanostructured substrates for super-resolution imaging (proceeding)
Maia Brunstein, Anne Talneau, Minh-Chau Nguyen, Pascal Berto, Anne-Laure Fehrembach, Anne Sentenac, Martin Oheim : Imaging Systems and Applications 2019, Munich Germany, 2019
Patents
- Calibration standard for evanescence microscopy
Martin Oheim, Adi Salomon : Patent # : WO 2020/053367 A1, 2020 - Micron-size plasmonic color sorter
A Salomon, A Weissman, E Segal : Patent # : PCT/IL2017/050756, (US 2018008029A1), 2016