winter-spring 2021, 2 Feb at 10h30, online – Ask link to a SPPIN member.
Dynamic imaging is an important tool for mapping the spatiotemporal activities in neurons and glial cells of the mammalian central nervous system. Wide-field fluorescent imaging enables ultrafast and truly synchronized acquisition of the focal plane image of specific signaling pathways. Multiple methods have been developed to endow wide-field microscopy with optical sectioning ability, so as to achieve high-clarity, high-speed and low phototoxicity imaging. In my presentation, I will talk about the application of wide-field single-photon light sheet microscopy (namely, selective planar illumination microscopy) in the study of mouse brain astrocyte activities, from a personal experimental point of view. The simplicity and high adaptability of light sheet microscopy provide more choices for a wide range of neuroscientific imaging studies.