11 March 2026 – Anne SENTENAC – RIM (super-resolution) random fluorescence microscopy

Anne SENTENAC – Equipe: SEMO, Institut Fresnel, Marseille

SPPIN’s seminar series winter-spring 2026

RIM (super-resolution) random fluorescence microscopy

Fluorescence microscopy plays a crucial role in biology due to its ability to provide functional images of living samples. Numerous techniques have been proposed in the last twenty years to improve its resolution beyond the 300 nm usually obtained with standard microscopes. Among them, the best compromise in terms of resolution and adaptation to live imaging is Structured Illumination Microscopy (SIM) in which several images of the sample are recorded under different patterned illuminations. SIM can use periodic illuminations as is periodic-SIM or focused spots as in Image Scanning Microscopy (ISM). Recently, we have developed an alternative to SIM called Random Illumination Microscopy (RIM) in which the illuminations are random speckles obtained by passing a laser beam through a diffuser. The major advantage of RIM compared to periodic-SIM and ISM is that it is insensitive to aberrations and scattering on the illumination side. It is thus more adapted to the imaging of aberrating samples. In my talk, I will present the general principles of super-resolved microscopy using structured illuminations and develop the RIM approach.

Please contact Marc Guillon if you want to make an appointment with Anne Sentenac