20 Mai 2025 – Alexandra FRAGOLA – Fast adaptive optics light-sheet microscopy for in vivo high-resolution imaging at depth

Prof Alexandra FRAGOLA, ISMO, Université Paris Saclay (Orsay)

SPPIN’s webinar 2024, 20 May at 11h, SPPIN common room, 3rd floor

Fast adaptive optics light-sheet microscopy for in vivo high-resolution imaging at depth

The study of fast biological phenomena at the cellular level has become possible even in depth in biological tissues in several animal models and rodents, thanks to efficient 3D microscopy techniques. Light sheet fluorescence microscopy (LSFM) is particularly well suited to imaging relatively transparent specimens such as zebrafish, but image quality deep inside biological tissues remains limited by scattering and optical aberrations due to the refractive index inhomogeneity, which limit both resolution and sensitivity in all microscopy modalities.