{"id":8,"date":"2023-01-23T16:02:12","date_gmt":"2023-01-23T15:02:12","guid":{"rendered":"https:\/\/www.sppin.fr\/multiscale\/?page_id=8"},"modified":"2023-06-29T17:09:37","modified_gmt":"2023-06-29T15:09:37","slug":"accueil","status":"publish","type":"page","link":"https:\/\/www.sppin.fr\/multiscale\/","title":{"rendered":"Idea & aim"},"content":{"rendered":"\n
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Transverse research lines of the SPPIN are collaborative research efforts in which at least three labs of the SPPIN team up to provide a shared research infrastructure that could not easily be bought or maintained by a single lab. At present, these efforts include, i- the M-Cube light-sheet mesoscope, the preparation of hIPSC organoids and the development of a universal, extremely rapid and non-noxious tissue clearing technique. This website gives a work-in-progress view of developments combining these three axes, under the umbrella term \u201cMultiscale neuroanatomy\u201d. Stay tuned.<\/p>\n<\/div>\n<\/div>\n\n\n\n

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Tissue clearing techniques, along with progress in 3-D fluorescence imaging have prompted a revival of neuroanatomy. The workhorse in such studies has been the light-sheet microscope, the defining feature of which is a crossed arrangement of excitation and collection optics. This arrangement brings about a trade-off between field-of-view against spatial resolution. Brain structures, however, span spatial scales of more than 6 orders of magnitude \u2014 from blood vessels and neuronal networks linking areas centimeters apart to tiny compartments like dendritic spine shafts or astrocyte peripheral processes that measure little more than a few tens of nm. Imaging such diverse structures in a continuous manner, from overviews to sub-\u00b5m detail would be extremely useful, but it would require a continuous adaptation in the illumination and collection optics that is difficult to implement on a single instrument. We built a compact, modular light-sheet microscope designed for correlative micro- meso- and macroscopic observation (M-Cube).<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Transverse research lines of the SPPIN are collaborative research efforts in which at least three labs of the SPPIN team up to provide a shared research infrastructure that could not easily be bought or maintained by a single lab. At present, these efforts include, i- the M-Cube light-sheet mesoscope, the preparation of hIPSC organoids and … <\/p>\n

Continuer la lecture de « Idea & aim »<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_themeisle_gutenberg_block_has_review":false,"footnotes":""},"class_list":["post-8","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.sppin.fr\/multiscale\/wp-json\/wp\/v2\/pages\/8","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sppin.fr\/multiscale\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.sppin.fr\/multiscale\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.sppin.fr\/multiscale\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.sppin.fr\/multiscale\/wp-json\/wp\/v2\/comments?post=8"}],"version-history":[{"count":11,"href":"https:\/\/www.sppin.fr\/multiscale\/wp-json\/wp\/v2\/pages\/8\/revisions"}],"predecessor-version":[{"id":153,"href":"https:\/\/www.sppin.fr\/multiscale\/wp-json\/wp\/v2\/pages\/8\/revisions\/153"}],"wp:attachment":[{"href":"https:\/\/www.sppin.fr\/multiscale\/wp-json\/wp\/v2\/media?parent=8"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}