Several SPPIN research teams use optical and electrophysiological methods aimed at understanding brain functions. In order to complete these studies, I provide morphological data and analysis. This includes probing the expression of certain proteins (receptors, ligands, channels) using confocal microscopy and immunohistochemistry techniques, or tracing the connections between neurons expressing fluorescent proteins. These morphological descriptions, together with the data obtained by electrophysiology, help to construct coherent explanations for the phenomena observed by the researchers.

Main activities

Mapping the interactions between different brain regions by tracing signals of fluorescent proteins expressed in genetically defined neuronal cell-types labeled by stereotactically delivered adeno-associated viruses. To accomplish this I have developed tissue clearing techniques, three-dimensional imaging infrastructure and data processing to image through tissue sections of 1-3mm thickness and whole brains up to 8mm in thickness.

Morphological study of neurons: I develop and conduct immunohistochemistry techniques on brain slices to explore the expression of certain proteins (receptors, ligands, channels), or to study the networks formed by different brain cells.

Participation in the publications of research data or technological achievements in scientific journals.

Recent Publications

Articles

• KCa1.1 channels contribute to optogenetically driven post-stimulation silencing in cerebellar molecular layer interneurons.
  Kassa M, Bradley J, Jalil A, Llano I : J Gen Physiol, 2023
• Synergism of type 1 metabotropic and ionotropic glutamate receptors in cerebellar molecular layer interneurons in vivo.
  Bao J, Graupner M, Astorga G, Collin T, Jalil A, Indriati DW, Bradley J, Shigemoto R, Llano I : Elife, 2020
• Influence of spatially segregated IP-producing pathways on spike generation and transmitter release in Purkinje cell axons.
  Gomez LC, Kawaguchi SY, Collin T, Jalil A, Gomez MDP, Nasi E, Marty A, Llano I : Proc Natl Acad Sci U S A, 2020
• Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice.
  Villette V, Chavarha M, Dimov IK, Bradley J, Pradhan L, Mathieu B, Evans SW, Chamberland S, Shi D, Yang R, Kim BB, Ayon A, Jalil A, St-Pierre F, Schnitzer MJ, Bi G, Toth K, Ding J, Dieudonné S, Lin MZ : Cell, 2019
• Expression and cellular function of vSNARE proteins in brain astrocytes.
  Ropert N, Jalil A, Li D : Neuroscience, 2015
• Impact of single-site axonal GABAergic synaptic events on cerebellar interneuron activity.
  Zorrilla de San Martín J, Jalil A, Trigo FF : J Gen Physiol, 2015
• An excitatory GABA loop operating in vivo.
  Astorga G, Bao J, Marty A, Augustine GJ, Franconville R, Jalil A, Bradley J, Llano I : Front Cell Neurosci, 2015
• Regulation of gap junctions in melanoma and their impact on Melan-A/MART-1-specific CD8⁺ T lymphocyte emergence.
  Benlalam H, Carré T, Jalil A, Noman Z, Caillou B, Vielh P, Tittarelli A, Robert C, Chouaib S : J Mol Med (Berl), 2013
• Attenuation of soft-tissue sarcomas resistance to the cytotoxic action of TNF-α by restoring p53 function.
  Muret J, Hasmim M, Stasik I, Jalil A, Mallavialle A, Nanbakhsh A, Lacroix L, Billot K, Baud V, Thiery J, Vielh P, Terrier P, Wiels J, Vassilev L, Lecesne A, Bonvalot S, Chouaib S : PLoS One, 2012
• RUNX1-induced silencing of non-muscle myosin heavy chain IIB contributes to megakaryocyte polyploidization.
  Lordier L, Bluteau D, Jalil A, Legrand C, Pan J, Rameau P, Jouni D, Bluteau O, Mercher T, Leon C, Gachet C, Debili N, Vainchenker W, Raslova H, Chang Y : Nat Commun, 2012
• Presence of a defect in karyokinesis during megakaryocyte endomitosis.
  Lordier L, Pan J, Naim V, Jalil A, Badirou I, Rameau P, Larghero J, Debili N, Rosselli F, Vainchenker W, Chang Y : Cell Cycle, 2012
• Minimal engagement of CD103 on cytotoxic T lymphocytes with an E-cadherin-Fc molecule triggers lytic granule polarization via a phospholipase Cgamma-dependent pathway.
  Le Floc\\’h A, Jalil A, Franciszkiewicz K, Validire P, Vergnon I, Mami-Chouaib F : Cancer Res, 2011
• hSMG-1 is a granzyme B-associated stress-responsive protein kinase.
  Meslin F, Hamaï A, Mlecnik B, Rosselli F, Richon C, Jalil A, Wemhoff G, Thiery J, Galon J, Chouaib S : J Mol Med (Berl), 2011
• Aurora B is dispensable for megakaryocyte polyploidization, but contributes to the endomitotic process.
  Lordier L, Chang Y, Jalil A, Aurade F, Garçon L, Lécluse Y, Larbret F, Kawashima T, Kitamura T, Larghero J, Debili N, Vainchenker W : Blood, 2010
• Gap junction communication between autologous endothelial and tumor cells induce cross-recognition and elimination by specific CTL.
  Benlalam H, Jalil A, Hasmim M, Pang B, Tamouza R, Mitterrand M, Godet Y, Lamerant N, Robert C, Avril MF, Neefjes J, Tursz T, Mami-Chouaib F, Kieda C, Chouaib S : J Immunol, 2009
• The cooperative induction of hypoxia-inducible factor-1 alpha and STAT3 during hypoxia induced an impairment of tumor susceptibility to CTL-mediated cell lysis.
  Noman MZ, Buart S, Van Pelt J, Richon C, Hasmim M, Leleu N, Suchorska WM, Jalil A, Lecluse Y, El Hage F, Giuliani M, Pichon C, Azzarone B, Mazure N, Romero P, Mami-Chouaib F, Chouaib S : J Immunol, 2009
• Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
  Franciszkiewicz K, Le Floc\\’h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadière C, Mami-Chouaib F : Cancer Res, 2009
• Trans-presentation of IL-15 dictates IFN-producing killer dendritic cells effector functions.
  Ullrich E, Bonmort M, Mignot G, Jacobs B, Bosisio D, Sozzani S, Jalil A, Louache F, Bulanova E, Geissman F, Ryffel B, Chaput N, Bulfone-Paus S, Zitvogel L : J Immunol, 2008
• Two opposite signaling outputs are driven by the KIR2DL1 receptor in human CD4+ T cells.
  Fourmentraux-Neves E, Jalil A, Da Rocha S, Pichon C, Chouaib S, Bismuth G, Caignard A : Blood, 2008
• Megakaryocyte endomitosis is a failure of late cytokinesis related to defects in the contractile ring and Rho/Rock signaling.
  Lordier L, Jalil A, Aurade F, Larbret F, Larghero J, Debili N, Vainchenker W, Chang Y : Blood, 2008
• Resistance of tumor cells to cytolytic T lymphocytes involves Rho-GTPases and focal adhesion kinase activation.
  Abouzahr-Rifai S, Hasmim M, Boukerche H, Hamelin J, Janji B, Jalil A, Kieda C, Mami-Chouaib F, Bertoglio J, Chouaib S : J Biol Chem, 2008
• ICAM-1 has a critical role in the regulation of metastatic melanoma tumor susceptibility to CTL lysis by interfering with PI3K/AKT pathway.
  Hamaï A, Meslin F, Benlalam H, Jalil A, Mehrpour M, Faure F, Lecluse Y, Vielh P, Avril MF, Robert C, Chouaib S : Cancer Res, 2008
• Alpha E beta 7 integrin interaction with E-cadherin promotes antitumor CTL activity by triggering lytic granule polarization and exocytosis.
  Le Floc\\’h A, Jalil A, Vergnon I, Le Maux Chansac B, Lazar V, Bismuth G, Chouaib S, Mami-Chouaib F : J Exp Med, 2007
• Membrane-bound interleukin (IL)-15 on renal tumor cells rescues natural killer cells from IL-2 starvation-induced apoptosis.
  Wittnebel S, Da Rocha S, Giron-Michel J, Jalil A, Opolon P, Escudier B, Validire P, Khawam K, Chouaib S, Azzarone B, Caignard A : Cancer Res, 2007
• Granzyme B-induced cell death involves induction of p53 tumor suppressor gene and its activation in tumor target cells.
  Meslin F, Thiery J, Richon C, Jalil A, Chouaib S : J Biol Chem, 2007
• Silencing of prion protein sensitizes breast adriamycin-resistant carcinoma cells to TRAIL-mediated cell death.
  Meslin F, Hamaï A, Gao P, Jalil A, Cahuzac N, Chouaib S, Mehrpour M : Cancer Res, 2007
• The decreased susceptibility of Bcr/Abl targets to NK cell-mediated lysis in response to imatinib mesylate involves modulation of NKG2D ligands, GM1 expression, and synapse formation.
  Cebo C, Da Rocha S, Wittnebel S, Turhan AG, Abdelali J, Caillat-Zucman S, Bourhis JH, Chouaib S, Caignard A : J Immunol, 2006
• Identification of target actin content and polymerization status as a mechanism of tumor resistance after cytolytic T lymphocyte pressure.
  Abouzahr S, Bismuth G, Gaudin C, Caroll O, Van Endert P, Jalil A, Dausset J, Vergnon I, Richon C, Kauffmann A, Galon J, Raposo G, Mami-Chouaib F, Chouaib S : Proc Natl Acad Sci U S A, 2006
• The decreased susceptibility of metastatic melanoma cells to killing involves an alteration of CTL reactivity.
  Gassara A, Messai Y, Gaudin C, Abouzahr S, Jalil A, Diarra-Mehrpour M, Faure F, Richon C, Avril MF, Even J, Chouaib S : Int J Oncol, 2006
• Deficiency in the Wiskott-Aldrich protein induces premature proplatelet formation and platelet production in the bone marrow compartment.
  Sabri S, Foudi A, Boukour S, Franc B, Charrier S, Jandrot-Perrus M, Farndale RW, Jalil A, Blundell MP, Cramer EM, Louache F, Debili N, Thrasher AJ, Vainchenker W : Blood, 2006
• Cytopathic effects of the cytomegalovirus-encoded apoptosis inhibitory protein vMIA.
  Poncet D, Pauleau AL, Szabadkai G, Vozza A, Scholz SR, Le Bras M, Brière JJ, Jalil A, Le Moigne R, Brenner C, Hahn G, Wittig I, Schägger H, Lemaire C, Bianchi K, Souquère S, Pierron G, Rustin P, Goldmacher VS, Rizzuto R, Palmieri F, Kroemer G : J Cell Biol, 2006
• Imatinib enhances human melanoma cell susceptibility to TRAIL-induced cell death: Relationship to Bcl-2 family and caspase activation.
  Hamaï A, Richon C, Meslin F, Faure F, Kauffmann A, Lecluse Y, Jalil A, Larue L, Avril MF, Chouaib S, Mehrpour M : Oncogene, 2006
• lyl-1 and tal-1/scl, two genes encoding closely related bHLH transcription factors, display highly overlapping expression patterns during cardiovascular and hematopoietic ontogeny.
  Giroux S, Kaushik AL, Capron C, Jalil A, Kelaidi C, Sablitzky F, Dumenil D, Albagli O, Godin I : Gene Expr Patterns, 2006
• p53 potentiation of tumor cell susceptibility to CTL involves Fas and mitochondrial pathways.
  Thiery J, Abouzahr S, Dorothee G, Jalil A, Richon C, Vergnon I, Mami-Chouaib F, Chouaib S : J Immunol, 2005
• Altered IFNgamma signaling and preserved susceptibility to activated natural killer cell-mediated lysis of BCR/ABL targets.
  Cebo C, Voutsadakis IA, Da Rocha S, Bourhis JH, Jalil A, Azzarone B, Turhan AG, Chelbi-Alix M, Chouaib S, Caignard A : Cancer Res, 2005
• The sensitivity of renal cell carcinoma cells to interferon alpha correlates with p53-induction and involves Bax.
  Wittnebel S, Jalil A, Thiery J, DaRocha S, Viey E, Escudier B, Chouaib S, Caignard A : Eur Cytokine Netw, 2005
• Opposite effects of estrogen receptors alpha and beta on MCF-7 sensitivity to the cytotoxic action of TNF and p53 activity.
  Lewandowski SA, Thiery J, Jalil A, Leclercq G, Szczylik C, Chouaib S : Oncogene, 2005
• The apoptosis/autophagy paradox: autophagic vacuolization before apoptotic death.
  González-Polo RA, Boya P, Pauleau AL, Jalil A, Larochette N, Souquère S, Eskelinen EL, Pierron G, Saftig P, Kroemer G : J Cell Sci, 2005
• Induction of CTGF by TGF-beta1 in normal and radiation enteritis human smooth muscle cells: Smad/Rho balance and therapeutic perspectives.
  Haydont V, Mathé D, Bourgier C, Abdelali J, Aigueperse J, Bourhis J, Vozenin-Brotons MC : Radiother Oncol, 2005
• Three pathways to mature macrophages in the early mouse yolk sac.
  Bertrand JY, Jalil A, Klaine M, Jung S, Cumano A, Godin I : Blood, 2005
• Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells.
  Zhang Y, Foudi A, Geay JF, Berthebaud M, Buet D, Jarrier P, Jalil A, Vainchenker W, Louache F : Stem Cells, 2004
• Prion protein prevents human breast carcinoma cell line from tumor necrosis factor alpha-induced cell death.
  Diarra-Mehrpour M, Arrabal S, Jalil A, Pinson X, Gaudin C, Piétu G, Pitaval A, Ripoche H, Eloit M, Dormont D, Chouaib S : Cancer Res, 2004
• Mutant alpha-actinin-4 promotes tumorigenicity and regulates cell motility of a human lung carcinoma.
  Menez J, Le Maux Chansac B, Dorothée G, Vergnon I, Jalil A, Carlier MF, Chouaib S, Mami-Chouaib F : Oncogene, 2004
• An anti-apoptotic viral protein that recruits Bax to mitochondria.
  Poncet D, Larochette N, Pauleau AL, Boya P, Jalil AA, Cartron PF, Vallette F, Schnebelen C, Bartle LM, Skaletskaya A, Boutolleau D, Martinou JC, Goldmacher VS, Kroemer G, Zamzami N : J Biol Chem, 2004
• NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs.
  Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, Ryffel B, Cambi A, Figdor C, Vainchenker W, Galy A, Caignard A, Zitvogel L : Blood, 2004
• Characterization of purified intraembryonic hematopoietic stem cells as a tool to define their site of origin.
  Bertrand JY, Giroux S, Golub R, Klaine M, Jalil A, Boucontet L, Godin I, Cumano A : Proc Natl Acad Sci U S A, 2004
• Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer.
  Debily MA, Camarca A, Ciullo M, Mayer C, El Marhomy S, Ba I, Jalil A, Anzisi A, Guardiola J, Piatier-Tonneau D : Hum Mol Genet, 2003
• Decreased production of local immunoglobulin A to Pneumocystis carinii in bronchoalveolar lavage fluid from human immunodeficiency virus-positive patients.
  Jalil A, Moja P, Lambert C, Perol M, Cotte L, Livrozet JM, Boibieux A, Vergnon JM, Lucht F, Tran R, Contini C, Genin C : Infect Immun, 2000
• Humoral immune response within the lung in HIV-1 infection.
  Moja P, Jalil A, Quesnel A, Perol M, Cotte L, Livrozet JM, Boibieux A, Chamson A, Vergnon JM, Lucht F, Tran R, Pozzetto B, Genin C : Clin Exp Immunol, 1997